Protein localization by fluorescence microscopy : a practical approach /
There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step t...
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| 格式: | Licensed eBooks |
| 语言: | 英语 |
| 出版: |
Oxford ; New York :
Oxford University Press,
©2000.
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| 丛编: | Practical approach series ;
218. |
| 在线阅读: | https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=98426 |
| 总结: | There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user |
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| 实物描述: | 1 online resource (xxiv, 231 pages) : illustrations (some color) |
| 参考书目: | Includes bibliographical references and index. |
| ISBN: | 0585483396 9780585483399 128037568X 9781280375682 9780191565915 0191565911 9786610375684 6610375682 0199637407 0199637415 |