MALDI mass spectrometry imaging : from fundamentals to spatial omics /

This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.

Detalhes bibliográficos
Outros Autores: Siegel, Tiffany (Editor)
Formato: Licensed eBooks
Idioma:inglês
Publicado em: London, UK : Royal Society of Chemistry [2022]
Colecção:New developments in mass spectrometry ; 12.
Acesso em linha:https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=3107815
Sumário:
  • Section 1
  • Chapter 1 An Introduction to MALDI Ionization Mechanisms for Users of Mass Spectrometry Imaging
  • 1.1 Introduction
  • 1.2 Laser Ablation in MALDI
  • 1.2.1 Laser Pulse Dependence
  • 1.2.2 Plume Pressure, Temperature and Velocity
  • 1.2.3 Laser Spot Size
  • 1.2.4 Local Thermal Equilibrium
  • 1.3 Primary Ionization
  • 1.3.1 Preformed Ions and "Lucky Survivors"
  • 1.3.2 Mechanical Charge Separation
  • 1.3.3 Thermal Ionization
  • 1.3.4 Non-thermal Ionization
  • 1.3.5 Metal Surfaces
  • 1.4 Secondary Ionization
  • 1.4.1 Matrix Suppression Effect
  • 1.4.2 Analyte Suppression Effect
  • 1.5 MALDI-2
  • 1.6 Conclusions
  • References
  • Chapter 2 MALDI Mass Spectrometry Imaging
  • Past, Present, and Future Challenges
  • 2.1 Opening Statement
  • 2.2 The Beginning
  • 2.3 The Last Two Decades
  • 2.3.1 MSI Data Acquisition and Instrumentation
  • 2.3.2 Automated Matrix Deposition
  • 2.3.3 On-tissue Chemistries
  • 2.3.4 Imaging of Xenobiotics
  • 2.4 Future Challenges
  • 2.4.1 The Push for Higher Spatial Resolution
  • 2.4.2 Standard Protocols and Methods
  • 2.4.3 Increasing Sensitivity and Specificity
  • 2.5 Concluding Remarks
  • References
  • Chapter 3 Instrumentation for MALDI-MSI
  • Part I Ionization Sources and Design
  • 3.1 Introduction
  • 3.2 Pressure Regime
  • 3.2.1 Plume Development
  • 3.2.2 Vacuum Ion Sources
  • 3.2.3 Intermediate Pressure Ion Sources
  • 3.2.4 Atmospheric Pressure Ion Sources
  • 3.3 Spatial Resolution in MALDI-MSI
  • 3.4 Optical Setups
  • 3.4.1 Front Side Illumination
  • 3.4.2 Transmission Mode Illumination
  • 3.5 Post-ionization
  • 3.5.1 MALDI-2
  • 3.5.2 MALDESI/LAESI
  • 3.5.3 Alternative Approaches
  • 3.6 Conclusions and Future Directions
  • References
  • Chapter 4 Instrumentation for MALDI-MSI
  • Part II Detection Systems
  • 4.1 Introduction
  • 4.2 Mass Analyzers and Their Performance for MALDI Imaging
  • 4.2.1 Sector Analyzers
  • 4.2.2 Quadrupole Mass Filters
  • 4.2.3 Time-of-flight
  • 4.2.4 QTOF
  • 4.2.5 Ion Traps
  • 4.2.6 Triple Quadrupoles
  • 4.2.7 FT-ICR
  • 4.2.8 Orbitrap
  • 4.3 Ion Mobility Spectrometry for Improved MALDI-MSI Performance
  • 4.3.1 Drift Tube Ion Mobility Spectrometry
  • 4.3.2 Traveling Wave Ion Mobility Spectrometry
  • 4.3.3 Trapped Ion Mobility Spectrometry
  • 4.3.4 High-field Asymmetric Waveform Ion Mobility
  • 4.4 Conclusion and Future Perspectives
  • Abbreviations
  • Acknowledgements
  • References
  • Chapter 5 Sample Preparation of Biological Tissues for MALDI-MSI
  • 5.1 Introduction
  • 5.2 Sample Collection
  • 5.3 Embedding and Storage
  • 5.4 Sectioning
  • 5.4.1 Frozen Material
  • 5.4.2 FFPE Material
  • 5.4.3 Important Considerations for Tissue Sectioning
  • 5.5 Washing
  • 5.5.1 Metabolites
  • 5.5.2 Lipids
  • 5.5.3 Proteins, Glycans and Peptides
  • 5.6 Enzymatic Digestion
  • 5.6.1 Proteins
  • 5.6.2 Glycans
  • 5.7 Derivatization
  • 5.7.1 Reagent Selection

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