MALDI mass spectrometry imaging : from fundamentals to spatial omics /

This book gathers knowledge about matrix-assisted laser desorption ionisation (MALDI) mass spectrometry imaging for postgraduate and professional researchers in academia and in industry where it has direct application to clinical research.

Xehetasun bibliografikoak
Beste egile batzuk: Siegel, Tiffany (Argitaratzailea)
Formatua: Licensed eBooks
Hizkuntza:ingelesa
Argitaratua: London, UK : Royal Society of Chemistry [2022]
Saila:New developments in mass spectrometry ; 12.
Sarrera elektronikoa:https://search.ebscohost.com/login.aspx?direct=true&scope=site&db=nlebk&AN=3107815
Aurkibidea:
  • Section 1
  • Chapter 1 An Introduction to MALDI Ionization Mechanisms for Users of Mass Spectrometry Imaging
  • 1.1 Introduction
  • 1.2 Laser Ablation in MALDI
  • 1.2.1 Laser Pulse Dependence
  • 1.2.2 Plume Pressure, Temperature and Velocity
  • 1.2.3 Laser Spot Size
  • 1.2.4 Local Thermal Equilibrium
  • 1.3 Primary Ionization
  • 1.3.1 Preformed Ions and "Lucky Survivors"
  • 1.3.2 Mechanical Charge Separation
  • 1.3.3 Thermal Ionization
  • 1.3.4 Non-thermal Ionization
  • 1.3.5 Metal Surfaces
  • 1.4 Secondary Ionization
  • 1.4.1 Matrix Suppression Effect
  • 1.4.2 Analyte Suppression Effect
  • 1.5 MALDI-2
  • 1.6 Conclusions
  • References
  • Chapter 2 MALDI Mass Spectrometry Imaging
  • Past, Present, and Future Challenges
  • 2.1 Opening Statement
  • 2.2 The Beginning
  • 2.3 The Last Two Decades
  • 2.3.1 MSI Data Acquisition and Instrumentation
  • 2.3.2 Automated Matrix Deposition
  • 2.3.3 On-tissue Chemistries
  • 2.3.4 Imaging of Xenobiotics
  • 2.4 Future Challenges
  • 2.4.1 The Push for Higher Spatial Resolution
  • 2.4.2 Standard Protocols and Methods
  • 2.4.3 Increasing Sensitivity and Specificity
  • 2.5 Concluding Remarks
  • References
  • Chapter 3 Instrumentation for MALDI-MSI
  • Part I Ionization Sources and Design
  • 3.1 Introduction
  • 3.2 Pressure Regime
  • 3.2.1 Plume Development
  • 3.2.2 Vacuum Ion Sources
  • 3.2.3 Intermediate Pressure Ion Sources
  • 3.2.4 Atmospheric Pressure Ion Sources
  • 3.3 Spatial Resolution in MALDI-MSI
  • 3.4 Optical Setups
  • 3.4.1 Front Side Illumination
  • 3.4.2 Transmission Mode Illumination
  • 3.5 Post-ionization
  • 3.5.1 MALDI-2
  • 3.5.2 MALDESI/LAESI
  • 3.5.3 Alternative Approaches
  • 3.6 Conclusions and Future Directions
  • References
  • Chapter 4 Instrumentation for MALDI-MSI
  • Part II Detection Systems
  • 4.1 Introduction
  • 4.2 Mass Analyzers and Their Performance for MALDI Imaging
  • 4.2.1 Sector Analyzers
  • 4.2.2 Quadrupole Mass Filters
  • 4.2.3 Time-of-flight
  • 4.2.4 QTOF
  • 4.2.5 Ion Traps
  • 4.2.6 Triple Quadrupoles
  • 4.2.7 FT-ICR
  • 4.2.8 Orbitrap
  • 4.3 Ion Mobility Spectrometry for Improved MALDI-MSI Performance
  • 4.3.1 Drift Tube Ion Mobility Spectrometry
  • 4.3.2 Traveling Wave Ion Mobility Spectrometry
  • 4.3.3 Trapped Ion Mobility Spectrometry
  • 4.3.4 High-field Asymmetric Waveform Ion Mobility
  • 4.4 Conclusion and Future Perspectives
  • Abbreviations
  • Acknowledgements
  • References
  • Chapter 5 Sample Preparation of Biological Tissues for MALDI-MSI
  • 5.1 Introduction
  • 5.2 Sample Collection
  • 5.3 Embedding and Storage
  • 5.4 Sectioning
  • 5.4.1 Frozen Material
  • 5.4.2 FFPE Material
  • 5.4.3 Important Considerations for Tissue Sectioning
  • 5.5 Washing
  • 5.5.1 Metabolites
  • 5.5.2 Lipids
  • 5.5.3 Proteins, Glycans and Peptides
  • 5.6 Enzymatic Digestion
  • 5.6.1 Proteins
  • 5.6.2 Glycans
  • 5.7 Derivatization
  • 5.7.1 Reagent Selection

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