TY - GEN T1 - Protein localization by fluorescence microscopy : a practical approach T2 - Practical approach series ; A2 - Allan, Victoria J. LA - English PP - Oxford ; New York PB - Oxford University Press YR - 2000 UL - https://ebooks.jgu.edu.in/Record/ebsco_acadsubs_ocm53956459 AB - There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is a vital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances the advantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solid grounding to the novice whilst extending the range of the experienced user OP - 231 CN - QP551 .P6965 2000eb SN - 0585483396 SN - 9780585483399 SN - 128037568X SN - 9781280375682 SN - 9780191565915 SN - 0191565911 SN - 9786610375684 SN - 6610375682 SN - 0199637407 SN - 0199637415 KW - Proteins : Analysis. KW - Fluorescence microscopy. KW - Green fluorescent protein. KW - Immunofluorescence. KW - Luminescent Proteins KW - Microscopy, Fluorescence KW - Sequence Analysis, Protein KW - Protéines : Analyse. KW - Microscopie de fluorescence. KW - Protéine fluorescente verte. KW - Photoprotéines. KW - fluorescence microscopy. KW - SCIENCE : Life Sciences : Biochemistry. KW - Sequence Analysis, Protein. KW - Microscopy, Fluorescence. KW - Luminescent Proteins. KW - Fluorescence microscopy KW - Green fluorescent protein KW - Immunofluorescence KW - Proteins : Analysis ER -